ATCC Medium: 2755 Desulfovibrio Medium
Solution A
K2HPO4……………………………………..…0.5 g
NH4Cl……………………………………….…1.0 g
Na2SO4………………………………….……1.0 g
CaCl2 x 2 H2O…………………………….….0.1 g
MgSO4 x 7 H2O………………………….…..2.0 g
DL-Na-lactate…………………………….……2.0 g
Yeast Extract………………………………….1.0 g
Resazurin………………………………………1.0 mg
DI Water……………………………………….980.0 ml
Solution B
FeSO4 x 7 H2O………………………………0.5 g
DI Water………………………………………10.0 ml
Solution C
Na-thioglycolate………………………………0.1 g
Ascorbic acid…………………………………0.1 g
DI Water………………………………………10.0 ml
Dissolve the ingredients of each solution in the appropriate quantities of water. Bring Solution A to a boil for a few minutes. Autoclave Solution A at 121?C and cool to room temperature while gassing with oxygen-free N2 gas. Filter sterilize Solution B and Solution C. Add both filtered solutions to Solution A and continue to gas the complete media. Adjust pH to 7.8 with NaOH, and distribute under N2 in anaerobic tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended.