固定电话：010-53515223

业务手机：18610886853,18610241664,18701099593,18971386853,18701098095

微信号码：vrmte521（18610886853企业微信）；18610241664；biobw0905（18701098095

企业公众号：800185668

官方邮箱：biobw11@sina.com（微生物菌种查询网）

投诉电话：biobw8888（18701099593）

微生物菌种资源

1、用户在微生物菌种查询网订购时请仔细确认订单后再下单，因菌株特殊性由用户选择而造成的退货需求，我单位不予受理（特殊情况除外）。

2、用户在收到菌株后请立即核验菌株包裹，若发现任何包装规格、数量、名称不符，外观破损，损毁等问题请在3个工作日内反馈给我单位业务人员并会及时处理上述问题。

3、用户收到快递包裹后，如果有漏发或发错货的情况，请在用户签收包裹之日起3个工作日内，将破损或发错的货品、加盖公章的换货申请表、邮运公司人员在场的开箱视频以及出库清单复印件一起寄给我们，可以顺丰到付。我们将在收到后，尽快核实并予以处理。

4、因微生物菌株属于活的生物制品如冻干常温运输4°C冰箱保存2-20年；如斜面培养物则用户收到后尽快实验。如西林瓶请置于-20°C可以保藏5-10年。如甘油管请置于-80°C并定期转接以确保其活性。

5、提交订单，一定要写菌株的编号，如不写，视为认可提供的该属种的任意菌株；提供的菌株，均只提供菌株打管说明或者菌株证明于网站下载；美国ATCC菌株绝大多数有COA；特声明告知，如再因以上情况引起异议与反悔，概不退菌。

6、已经确认退货的话用户需把运单号及时告知，且与我单位客服联系退款换货事宜。

细胞系原代细胞株

1、用户在中国微生物菌种查询网订购时请仔细确认订单后再下单，因细胞特殊性由用户选择而造成的退货需求，我单位不予受理（特殊情况除外）。

2、细胞运输途中遭遇的各种问题，细胞丢失、瓶身破损、培养液严重漏液等，重发；细胞污染问题，请在收到产品24小时内，给我们提出真实的实验结果，核实后重发等。

3、其他因细胞引起的需要重发补发可以参考单位网站《细胞售后服务告知书》栏目。

4、已经确认退货的话用户需把运单号及时告知，且与我单位客服联系退款或者换货事宜。

其他产品

1、用户在中国微生物菌种查询网订购时请仔细确认订单后再下单，因产品特殊性由用户选择而造成的退货需求，我单位不予受理（特殊情况除外）。

2、产品在运输途中遭遇的各种问题，产品丢失、瓶身破损、漏液等，均可申请退换货。

3、用户收到快递包裹后，如果有漏发或发错货的情况，请在用户签收包裹之日起3个工作日内，将破损或发错的货品、加盖公章的换货申请表、邮运公司人员在场的开箱视频以及出库清单复印件一起寄给我们，可以顺丰到付。我们将在收到后，尽快核实并予以处理。

4、已经确认退货的话用户需把运单号及时告知，且与我单位客服联系退款换货事宜。

Klebsiella pneumoniae subsp. pneumoniae (Schroeter) Trevisan拉丁名

(ATCC? 700603?) 统一编号

Strain Designations 菌种别名: K6 [CCUG 45421, LMG 20218, MCV37]  / 

Type Strain 模式菌种: no  / 

Biosafety Level 生物安全等级 : 2

Deposited As Klebsiella pneumoniae (Schroeter) Trevisan

Application 用途Produces beta-lactamase SHV-18

Quality control strain

Resistant to ampicillin

Resistant to aztreonam

Resistant to cefoxitin

Resistant to cefpodoxime

Resistant to ceftazidime

Resistant to chloramphenicol

Resistant to piperacillin

Resistant to tetracycline

Respiratory research

Sensitive to amoxicillin-clavulanate

Sensitive to cefepime

Intermediate resistance for cefotaxime

Sensitive to ciprofloxacin

Sensitive to imipenem

Sensitive to piperacillin-tazobactam

Intermediate resistance for Tobramycin

Sensitive to trimethoprim-sulfamethoxazole

Susceptibility disc testing

Susceptibility testing

Control for extended-spectrum beta-lactamase production
Isolation Urine from hospitalized patient, Richmond, VA, 1994

Product Format 提供形式 freeze-dried

Storage Conditions保藏方法

Frozen（冷冻物）: -80℃ or colder
Freeze-Dried（冻干物）: 2℃ to 8℃
Live Culture（活物）: See Propagation Section 
Preceptrol? no

Type Strain 模式菌种no 

Antibiotic ResistanceResistant to cefoxitin
Resistant to cefpodoxime
Resistant to ceftazidime
Resistant to chloramphenicol
Resistant to piperacillin
Resistant to ceftriaxone
Sensitive to trimethoprim-sulfamethoxazole
Sensitive to imipenem
Resistant to aztreonam
Resistant to ampicillin
Resistant to tetracycline
Sensitive to amoxicillin-clavulanate
Sensitive to cefepime
Sensitive to cefotaxime
Sensitive to ciprofloxacin
Sensitive to piperacillin-tazobactam
Intermediate resistance to gentamicin
Comments MIC (?g/mL):  Ampicillin >64 Amoxicillin-clavulanate 8
Aztreonam 64 Aztreonam-clavulanate <=0.25
Cefotaxime 8  Cefotaxime-clavulanate <=0.25
Cefpodoxime 16 Cefpodoxime-clavulanate 1 Ceftazidime 32
Ceftazidime-clavulanate 1
Ceftriaxone 16
Ceftriaxone-clavulanate <=0.25
Cefepime 1
Cefoxitin 32
Chloramphenicol >32
Ciprofloxacin 0.5
Gentamicin 8
Imipenem <=1
Piperacillin >128
Piperacillin-tazobactam 16 Tetracycline 16 Tobramycin 4

Trimethoprim-sulfamethoxazole

Medium 培养基 ATCC? Medium 3: Nutrient agar or nutrient broth

Growth Conditions生长条件

Temperature 培养温度: 37℃

Atmosphere 需氧情况 : Aerobic

Name of Depositor 寄存人 JM Swenson

Special Collection NCRR

ContractChain of Custody ATCC <-- JM Swenson <-- MJ Ferraro <-- G Jacoby <-- H Dalton
Isolation Urine from hospitalized patient, Richmond, VA, 1994
References
Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9.

Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8.

Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M31-A3.

Rasheed JK, et al. Characterization of the extended-spectrum beta-lactamase reference strain, Klebsiella pneumoniae K6 (ATCC 700603), which produces the novel enzyme SHV-18. Antimicrob. Agents Chemother. 44: 2382-2388, 2000. PubMed: 10952583

 Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M100-S20, 2007

BD Phoenix Gram Negative AST NMIC/ID QC Set. BD Becton Dickinson; Phoenix

MicroScan Dried Gram Negative Panel QC Set. Dade/Siemens.

Sensititre? MIC Susceptibility. Thermo Scientific? Sensititre? Systems, Trek Diagnostic Systems.

Jana M Swenson, personal communication

VITEK 2 AST-GN QC Set. bioMerieux.

MicroScan ESBL plus QC Set. Dade/Siemens.

MicroScan Synergies plus Gram Negative QC Set. Dade/Siemens.

ETEST ESBL STRIP. bioMerieux.

Cross References

Nucleotide (GenBank) : AX111170 Sequence 1903 from Patent WO0123604.

Nucleotide (GenBank) : AF132290 Klebsiella pneumoniae plasmid-encoded extended-spectrum beta-lactamase (bla SHV-18) gene, complete cds.