ATCC medium: 928 Nitrosococcus medium
Ammonium sulfate.........................1.32 g
MgSO4 . 7H2O ...........................380.0 mg
CaCl2 . 2H2O ............................20.0 mg
Chelated iron (Geigy Chem 13% iron)......1.0 mg
Na2MoO4 . 2H2O..........................100.0 mcg
MnCl2 . 4H2O ...........................200.0 mcg
CoCl2 . 6H2O .............................2.0 mcg
ZnSO4 . 7H2O ...........................100.0 mcg
K2HPO4 ...................................8.7 mg
Phenol red 0.04%.........................3.25 ml
Seawater (filtered)......................1.0 L
Adjust final pH to 7.5-7.8 with 1 N HCl. Autoclave at 121C for 15 minutes.