ATCC medium: 481 Nitrobacter medium 204
Solution A (see below).......0.5 ml
Solution B (see below).......0.5 ml
Solution C (see below).......1.0 ml
Solution D (see below).......0.5 ml
Solution E (see below).......0.5 ml
Solution F (see below).......2.0 drops
Seawater...................700.0 ml
Distilled water to...........1.0 L
Solution A:
CaCl2 ........................2.0 g
Distilled water............100.0 ml
Solution B:
MgSO4 . 7H2O ................20.0 g
Distilled water............100.0 ml
Solution C:
EDTA.........................0.14 g
FeSO4 . 7H2O .................0.5 g
H2SO4 (conc.) ................0.05 ml
Distilled water............100.0 ml
Solution D (trace metals):
Na2MoO4 . 2H2O................0.1 g
MnCl2 . 4H2O .................0.2 g
CoCl2 . 6H2O .................0.002 g
ZnSO4 . 7H2O .................0.1 g
CuSO4 . 5H2O .................0.02 g
Distilled water..............1.0 L
These ingredients should be dissolved separately and then added together to make 1.0 L.
Solution E:
NaNO2 .......................41.4 g
Distilled water............100.0 ml
Solution F:
K2HPO4 .......................1.74 g
Distilled water............100.0 ml