ATCC medium: 2265 Nitrosomonas europaea medium
Solution 1:
(NH4)2SO4 (for 50 mM NH4 + ) ................................................. 4.95 g
KH2PO4 ................................................................................... 0.62 g
MgSO4 . 7H2O ......................................................................... 0.27 g
CaCl2 . 2H2O ........................................................................... 0.04 g
FeSO4 (30 mM in 50 mM EDTA at pH 7.0) ........................... 0.5 m
l CuSO4 . 5H2O.......................................................................... 0.2 mg
Distilled water ......................................................................... 1.2 L
Filter sterilize.
Solution 2:
KH2PO4 ................................................................................... 8.2 g
NaH2PO4.................................................................................. 0.7 g
Distilled water ..................................................................... 300.0 ml
Bring to pH 8.0 with 10N NaOH. Filter sterilize.
Solution 3 (buffer): Na2CO3 anhydrous................................................................... 0.6 g
Distilled water ....................................................................... 12.0 ml
Filter sterilize.
Complete medium: Combine Solutions 1, 2 and 3. Dispense aseptically into desired aliquots.