ATCC幽门螺杆菌（Helicobacter pylori，Hp）是一种专性微需氧菌。它的稳定生长需要依靠在生长的微环境中含2-8%的O2。因此，它在大气中和绝对厌氧条件下均不能生长。从临床标本中分离野生株都必需补充适量的CO2。而且它生长缓慢，通常需要3-5d，甚至更长时间，才能形成针尖状小菌落。为了避免快速生长的兼性厌O2菌和霉菌等的过度生长，常需加入由万古霉素、TMP、两性霉素、多粘菌素等组合的抑菌剂。 幽门螺杆菌在液体培养基中生长更加困难。这可能与在液体里面更难保证菌体周围稳定的微含O2量环境有关。 在血琼脂中，幽门螺杆菌不能在含有5%胆汁的情况下生长。将细菌暴露于含有5%胆汁的液体培养基中30min，有25%的幽门螺杆菌被杀死。这说明幽门螺杆菌通过十二指肠时尚有生存的可能性。
菌种名称：幽门螺杆菌
Helicobacter pylori (Marshall et al.) Goodwin et al. 拉丁名
(ATCC? 49503?) 统一编号
Strain Designations ATCC菌种别名 60190
Application 用途 Enteric Research；
Isolation 分离基物 Human gastric antrum
Biosafety Level 生物安全等级 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen
Storage Conditions 保藏条件
Frozen 冷冻物: -80℃ or colder
Freeze-Dried 冻干物: 2℃ to 8℃
Live Culture 活物: See Propagation Section
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Preceptrol? no
Type Strain 模式菌株 no
Comments 注释 Cytotoxin activity and serologic response
Medium 培养基 ATCC? Medium 2856: Brucella Agar/Broth w/ 10% FBS
ATCC? Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Growth Conditions 生长条件 Temperature 培养温度: 37℃
Atmosphere 需氧情况: Microaerophilic
Name of Depositor 寄存人 TL Cover U.S. Patent Number 5,618,840
Chain of Custody 来源国家 ATCC <-- TL Cover <-- R. Leunk <-- A. Pearson and P. Hawtin
References 参考文献 Cover TL, et al. Characterization of and human serologic response to proteins in Helicobacter pylori broth culture supernatants with vacuolizing cytotoxin activity. Infect. Immun. 58: 603-610, 1990. PubMed: 2307514
Cao P, Cover TL. High-level genetic diversity in the vapD chromosomal region of Helicobacter pylori. J. Bacteriol. 179: 2852-2856, 1997. PubMed: 9139899
Melchers K, et al. Cloning and membrane topology of a P type ATPase from Helicobacter pylori. J. Biol. Chem. 271: 446-457, 1996. PubMed: 8550601
Dubois A, et al. Transient and persistent experimental infection of nonhuman primates with Helicobacter pylori: implications for human disease. Infect. Immun. 64: 2885-2891, 1996. PubMed: 8757808
Cross References Nucleotide (GenBank) : Y08762 Helicobacter pylori flhA gene and unknown ORF.
Nucleotide (GenBank) : AC002058 Helicobacter pylori, *** SEQUENCING IN PROGRESS ***, in ordered pieces.
Nucleotide (GenBank) : U05676 Helicobacter pylori 60190 cysteinyl-tRNA synthetase homolog gene, partial cds, and vacuolating cytotoxin (vacA) gene, complete cds.