ATCC medium: 1352 Oxalate maintenance medium
K2HPO4 .......................0.25 g
KH2PO4 .......................0.25 g
(NH4)2SO4 ....................0.5 g
MgSO4 . 7H2O .................0.025 g
Sodium acetate...............0.82 g
Yeast extract................1.0 g
Trypticase (BD 211921).......1.0 g
Sodium oxalate...............5.0 g
Resazurin....................0.001 g
Na2CO3 .......................4.0 g
L-Cysteine . HCl.............0.5 g
Distilled water..............1.0 L
Mix ingredients except Na2CO3 and cysteine, adjust pH to 6.8, heat to boiling and then cool while gassing with O2-free CO2. Then add Na2CO3 and cysteine and tube under a CO2 atmosphere using Hungate or VPI Anaerobe Lab techniques. Cap with butyl rubber stoppers. Autoclave in press to hold stoppers on tubes. Transfer cultures under CO2 atmosphere.