ATCC medium: 1678 YGCP medium
Yeast extract......................2.5 g
Glucose...........................20.0 g
Peptone, Bacto (BD 211677).........2.5 g
NaCl...............................1.0 g
K2HPO4 .............................1.0 g
MgSO4 (anhydrous) ..................0.5 g
CaCO3 .............................10.0 g
Agar..............................17.5 g
Distilled water....................1.0 L
Add components except CaCO3 and adjust to pH 7.2. Add CaCO3 and autoclave at 121C for 15 minutes.