ATCC medium: 2584 Buffered Saltwater medium
HEPES (pH 7.7-7.8).......................4.77 g
Instant Ocean (75%).....................27.75 ml
Yeast extract............................0.05 g
Peptone..................................0.05 g
KHCO3....................................0.20 g
MnCl2....................................0.02 g
Distilled deionized water................1.0 L
Filter sterilize to avoid precipitation of the seawater. For solid media, split the volume of water in half and filter the portion that has the saltwater solution. Autoclave the agar separately.