ATCC medium: 1625 Super MMB
Peptone........................................0.40 g
Yeast extract..................................1.00 g
NH4Cl ..........................................0.20 g
CaCl2 . 2H2O ...................................0.01 g
NaCl...........................................0.20 g Mg
SO4 . 7H2O ...................................0.20 g
Ferric citrate.................................5.0 mg
Trace Elements Solution SL-6 (see below).......1.0 ml
Vitamin Solution (see below)..................20.0 ml
KH2PO4 .........................................1.0 g
Sodium succinate...............................0.4 g
Distilled water................................1.0 L
Adjust medium for final pH 7.0. Autoclave at 121C for 15 minutes.
Trace Elements Solution SL-6:
ZnSO4 . 7H2O .......................0.10 g
MnCl2 . 4H2O .......................0.03 g
H3BO3 ..............................0.3 g
CoCl2 . 6H2O .......................0.2 g
CuCl2 . 2H2O .......................0.01 g
NiCl2 . 6H2O .......................0.02 g
Na2MoO4 . 2H2O......................0.03 g
Distilled water....................1.0 L
Adjust final pH of Trace Elements Solution SL-6 to 3.4.
Vitamin Solution:
Biotin.............................2.0 mg
Folic acid.........................2.0 mg
Thiamine . HCl.....................5.0 mg
Calcium D-(+)-pantothenate.........5.0 mg
Vitamin B12........................0.1 mg
Riboflavin.........................5.0 mg
Distilled water....................1.0 L