ATCC Medium: 1065 LB Agar/Broth, Miller
Agar Medium
LB Agar (BD 244520)………………………..40 g
DI Water………………………………….……1000 ml
Autoclave at 121?C.
Broth Medium
LB Broth (BD 244620)………………..…….25.0 g
DI Water…………………………………..….1000 ml
Autoclave at 121?C.
*This product is commercially available from BD.
To make medium from scratch, follow formulation below:
LB Agar, Miller Composition
Tryptone……………………..…………..….10.0 g
Yeast Extract……..…………………….…...5.0 g
Sodium Chloride…………………………...10 g
Agar………………………………………….15.0 g
Final pH 7.0 +/- 0.2. *Omit agar for broth medium.