ATCC Medium: 2869 HV Medium
Humic acid……………………………………..1 g
KCl………………………………………………1.7 g
Na2HPO4………………………………………0.5 g
MgSO4…………………………………………0.5 g
CaCO3…………………………………………0.02 g
FeSO4…………………………………………0.01 g
VB Stock Solution (see below)……..……….1 ml
Agar (if necessary)……………………………15 g
DI Water……………………………………….1000 ml
Adjust pH to 7.2. Autoclave at 121C.
VB Stock Solution
VB1………………………………………….50 mg
VB2………………………………………….50 mg
Niacin……………………………………….50 mg
VB6………………………………………….50 mg
D-calcium pantothenate…………………..50 mg
Inositol………………………………………50 mg
PABA……………………………………….50 mg
Biotin………………………………………..25 mg
DI Water……………………………………100 ml