ATCC medium: 2235 PG medium for Halosimplex
NaCl.......................200.0 g
MgSO4 . 7H2O ................20.0 g
KCl..........................4.0 g
K2HPO4 .......................0.5 g
(NH4)2SO4.....................1.0 g
Sodium pyruvate*.............2.5 g
Glycerol.....................2.5 g
Distilled water..............1.0 L
*Sodium acetate may be used instead of sodium pyruvate. Adjust pH to 7.4. Autoclave at 121C for 15 minutes.