ATCC medium: 1804 NADC - 99X medium
Mineral Solution 1 (see below)....37.5 ml
Mineral Solution 2 (see below)....37.5 ml
Yeast extract......................1.0 g
Resazurin..........................1.0 mg
Arginine...........................2.11 g
Histidine..........................1.55 g
Phytone (BD 211906)................2.0 g
2,3 Dihydroxypyridine (optional)...0.25 g
Na2CO3 .............................4.0 g
L-Cysteine . HCl...................0.5 g
Distilled water to.................1.0 L
Add components except sodium carbonate and cysteine.
Adjust medium to pH 6.4. Boil and cool under 100% CO2.
Add sodium carbonate and cysteine. Tube medium anaerobically under same gas phase. Autoclave at 121C for 15 minutes.
Mineral Solution 1:
K2HPO4 .............................6.0 g
Distilled water....................1.0 L
Mineral Solution 2:
KH2PO4 .............................6.0 g
(NH4)2SO4..........................12.0 g
NaCl..............................12.0 g
MgSO4 . 7H2O .......................1.2 g
CaCl2 . 6H2O .......................1.2 g
Distilled water....................1.0 L