ATCC Medium: 96 Nitrobacter Medium B
NaNO2……………………………………………1.0 g
K2HPO4…………………………………………..0.5 g
NaCl……………………………………………….0.3 g
MgSO4……………………………………………0.5 g
MnSO4……………………………………………0.002 g
Fe2(SO4)3………………………………………..0.005 g
DI Water………………………………………….1000 ml
Adjust pH to 7.5. Dispense in 100 ml amounts in flasks.
Sterilize. Wash marble chips (Fisher M123-3) in distilled water.
Place several chips in 18 x 150 mm test tubes. Sterilize one hour. Aseptically add medium to cover the chips.