ATCC medium: 80 Lactic bacteria broth

中国微生物菌种查询网 / 2016-12-21 12:52:26

ATCC medium: 80 Lactic bacteria broth

Trypticase Peptone (BD 211921)..........10.0 g

Yeast autolysate.........................5.0 g

Sodium acetate..........................12.0 g

Glucose.................................10.0 g

Solution A (see below)...................5.0 ml
Solution B (see below)...................5.0 ml

Distilled water to.......................1.0 L

Adjust pH to 5.1-5.3.

Autoclave at 121C for 15 minutes.

Solution A: K2HPO4 ..................................10.0 %

KH2PO4 ..................................10.0 %

Solution B: MgSO4 ....................................4.0 %

NaCO.....................................0.2 %

FeSO4 ....................................0.2 %

MnSO4 ....................................0.2 %

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