ATCC medium: 1660 MH salts
NaCl...................................120.5 g
MgCl2 . 6H2O ............................22.4 g
MgSO4 ...................................14.4 g
CaCl2 ....................................0.54 g
KCl......................................3.0 g
NaHCO3 ...................................0.09 g
NaBr....................................39.0 mg
Proteose Peptone No. 3 (BD 211693).......5.0 g
Yeast extract...........................10.0 g
Glucose..................................1.0 g
Agar....................................20.0 g
Distilled water to.......................1.0 L
Adjust medium for final pH 7.5. Autoclave at 121C for 15 minutes.