ATCC medium: 566 Megasphaera medium
KH2PO4 .............................1.6 g
K2HPO4 .............................3.2 g
Sodium lactate (60% solution).....16.0 ml
Yeast extract......................4.0 g
NH4Cl ..............................0.5 g
CaCl2 ..............................0.2 g
MgCl2 ..............................0.2 g
Sodium thioglycollate..............0.45 g
Agar...............................1.0 g
Distilled water to.................1.0
L Adjust pH to 7.0. Autoclave at 121C for 15 minutes.