ATCC medium: 510 Acidaminococcus medium (VR)
Acid hydrolyzed casein, vitamin- and salt-free......20.0 g
Glucose..............................................5.0 g
VR Salts A (see below)..............................30.0 m
l VR Salts B (see below)...............................4.0 ml
L-Cysteine . HCl.....................................0.35 g
DL-Tryptophan........................................0.1 g
Guanine..............................................0.01 g
Uracil...............................................0.01 g
Hypoxanthine.........................................0.01 g
Pyridoxal............................................1.0 mg
Calcium D-(+)-pantothenate...........................1.0 mg
Thiamine............................................50.0 mcg
Niacin..............................................50.0 mcg
Riboflavin..........................................50.0 mcg
p-Aminobenzoic acid.................................10.0 mcg
Biotin...............................................2.0 mcg
Folic acid...........................................1.0 mcg
Vitamin B12..........................................1.0 mcg
Distilled water......................................1.0 L
Filter-sterilize vitamins as separate stock solution and add aseptically to sterile basal medium. Adjust medium, if necessary, to pH 7.0 with solid K2CO3. Prepare and dispense medium anaerobically using Hungate techniques and 100% N2 gas.
VR Salts A:
Na2HPO4 .....................37.5 g
KH2PO4 ......................12.5 g
H2O ........................500.0 ml