ATCC medium: 535 PYGM medium
Peptone..................................5.0 g
Yeast extract............................2.5 g
Glucose..................................1.25 g
Maltose..................................1.25 g
Cysteine.................................0.125 g
Salts Solution (see below)..............10.0 ml
Resazurin (0.025% aqueous solution)......1.0 ml
Distilled water........................250.0 ml
Autoclave at 121C for 15 minutes. Salts Solution:
K2HPO4 .......................0.1 g
KH2PO4 .......................0.1 g
NaHCO3 .......................1.0 g
NaCl.........................0.2 g
CaCl2 (anhydrous) ............0.02 g
MgSO4 ........................0.02 g
Na2MoO4 . 2H2O............Trace
CoCl2 . 6H2O .............Trace
50% H2SO4 ....................0.3 ml
Distilled water............100.0 ml