Glucose...........................10.0 g
Soluble starch.....................2.0 g
Peptone (Oxoid L37)................2.5 g
Yeast Extract (Oxoid L21)..........2.5 g
Neopeptone, DIFCO (BD 211681)......5.0 g
Trypticase Peptone (BD 211921).....2.0 g
1 M Phosphate buffer, pH 6.7......50.0 ml
L-Cysteine . HCl...................0.25 g
Distilled water..................950.0 ml
Adjust medium for final pH 7.2. Dispense medium into tubes flushed with 90% N2, 10% CO2 and immediately plug with butyl rubber stoppers. Autoclave at 121C for 15 minutes.