K2HPO4 ...................................1.0 g
MgSO4 . 7H2O .............................0.2 g
CaCl2 . 2H2O ............................20.0 mg
Na2S2O3 . 5H2O ............................0.2 g
Sodium pyruvate..........................2.2 g
Yeast extract............................4.0 g
(NH4)2SO4.................................1.0 g
Trace Elements Solution(see below)......1.0 ml
Chelated Iron Solution (see below).......2.0 ml
d-Biotin................................15.0 mcg
Vitamin B12.............................20.0 mcg
Distilled water..........................1.0 L
Adjust medium for final pH 6.9. Autoclave at 121C for 15 minutes.
Trace Elements Solution:
EDTA.........................2.5 mg
MnCl2 . 4H2O .................0.2 g
H3BO3 ........................0.1 g
Na2MoO4 . 2H2O................0.1 g
ZnCl2 .......................50.0 mg
NiCl2 . 6H2O ................50.0 mg
CoCl2 . 6H2O ................20.0 mg
CuCl2 . 2H2O .................5.0 mg
VOSO4 . 2H2O .................5.0 mg
Na2WO4 . 2H2O.................5.0 mg
Distilled water............250.0 ml
Chelated Iron Solution:
EDTA.........................1.0 g
FeCl3 . 6H2O .................0.5 g
Concentrated HCl.............1.5 ml
Distilled water..............1.0 L