ATCC medium: 1047 MN Marine medium (ATCC Medium 957) with 20 mcg/L of vitamin B12 ATCC Medium 957 (see below) with 20 mcg/L vitamin B12 added
ATCC Medium 957:
MgSO4 . 7H2O .......................... 0.04 g
CaCl2 . 2H2O .......................... 0.02 g
NaNO3 ................................. 0.75 g
K2HPO4 . 3H2O.......................... 0.02 g
Citric acid........................... 3.0 mg
Ferric ammonium citrate............... 3.0 mg
DTA.................................. 0.5 mg
Na2CO3 ................................ 0.02 g
Trace Metals A-5 (see below).......... 1.0 ml
Agar Noble (Difco 0142) (if needed).. 10.0 g
Distilled water..................... 250.0 ml
Seawater............................ 750.0 ml
Adjust pH after autoclaving to 8.5 with KOH.
Trace Metals A-5:
H3BO3 .......................... 2.86 g
MnCl2 . 4H2O ................... 1.81 g
ZnSO4 . 7H2O ................... 0.222 g
Na2MoO4 . 2H2O ................. 0.039 g
CuSO4 . 5H2O ................... 0.079 g
Co(NO3)2 . 6H2O................ 49.4 mg
Distilled water................ 1.0 L