ATCC medium: 472 Thiobacillus A2 (T3) medium
Solution A: Na2HPO4 ......................4.2 g
KH2PO4 .......................1.5 g
Na2S2O3 . 5H2O ................5.0 g
NH4Cl ........................1.0 g
Phenol red (0.2% soln.)......1.0 ml
Distilled water............100.0 ml
Adjust Solution A to pH 9.0.
Solution B:
MgSO4 . 7H2O .......................0.1 g
Trace Metal Solution (see below)...5.0 ml
Distilled water....................1.0 L
Sterilize Solutions A and B separately. Mix and dispense aseptically. Final pH will be 8.5 +/- 0.2. For solid medium, add 1.5% agar.
Trace Metal Solution:
EDTA........................50.0 g
ZnSO4 .......................22.0 g
CaCl2 ........................5.54 g
MnCl2 ........................5.06 g
FeSO4 ........................4.99 g
Ammonium molybdate...........1.10 g
CuSO4 ........................1.57 g
CoCl2 ........................1.61 g
Distilled water..............1.0 L
Adjust pH to 6.0 with KOH.