Yeast extract................2.0 g
Casein hydrolysate...........2.0 g
K2HPO4 .......................7.0 g
KH2PO4 .......................3.0 g
Sodium Citrate . 3H2O ........0.5 g
MgSO4 . 7H2O .................0.1 g
(NH4)2SO4.....................1.0 g
Glucose (filter-sterilized)..2.0 g
Agar........................15.0 g
Distilled water..............1.0 L
Adjust final pH to 7.0. Filter-sterilize glucose as 10% solution. Autoclave the basal medium at 121C for 15 minutes, and aseptically add the glucose for a final concentration of 0.2%.