ATCC Medium: 177 Fluid Thioglycollate Medium
Fluid Thioglycollate Medium (BD 211260)…….. 29.5 g
DI Water…………………………………………….1000 ml
Boil, dispense 8 mls into vessels.
Autoclave at 121?C.
*This product is commercially available from BD. To make medium from scratch, follow formulation below:
Yeast Extract……………………………………..5.0 g
Casitone Peptone………………………………15.0 g
Dextrose………………………………………….5.5 g
NaCl………………………………………………2.5 g
L-Cystine…………………………………………0.5 g
Sodium thioglycollate…………………………...0.5 g
Agar……………………………………………….0.75 g
Resazurin………………………………………..1.0 mg
DI Water………………………………………….1000 ml
Final pH 7.1 +/- 0.2.