ATCC Medium: 1115 Brucella albimi Broth
Broth Medium Brucella Broth (BD 211088)…………………….……….28.0 g
DI Water………………………………………………..….1000 ml
Autoclave at 121?C. *This product is commercially available from BD.
To make medium from scratch, follow formulation below:
Tryptone (Trypticase)………………………………..10.0 g
Peptamin *(Peptone)………………………………..10.0 g
Dextrose……………………………………………....1.0 g
Yeast Extract………………………………………….2.0 g
NaCl…………………………………………………...5.0 g
Na2S2O5...…………………………………………....0.1 g
DI Water……………………………………………….1000 ml
pH 7.0 +/- 0.2
*Peptic Digest of Animal Tissue