空肠弯曲菌（Campylobacter jejuni ）菌体轻度弯曲似逗点状，长1.5～5 μm,宽0.2～0.8 μm。菌体一端或两端有单鞭毛，运动活泼，在暗视野镜下观察似飞蝇。无荚膜，不形成芽胞。微需氧菌，在含5% O2或10% CO2和85% N2的环境中生长最好。最适生长温度42℃。在正常大气或无氧环境中均不能生长。
形态与染色
革兰阴性弯曲小杆菌，多种形态，有螺旋形、弯曲杆状、S形、逗点状、飞翔的海鸥形，陈旧培养物中可变成球形。无芽孢、无荚膜，有鞭毛，运动活泼，似飞箭样、梭样翻滚运动。
培养特性
微需氧：5％氧气、10％二氧化碳、85％氮气； 最适生长温度：42～43℃；
营养要求高，含血液和血清的培养基上生长良好，液体培养基混浊生长。
检测方法
用于弯曲菌检测的经典方法常用的为生化鉴定方法，但检测时间长、试剂成本高、尤其空肠弯曲菌的微需氧特性，使其分离培养更为困难，需要额外的维持微需氧条件的设施和试剂,近年来,PCR等分子生物学检测方法取得了长足发展,它具有敏感性高、特异性强、检测快速等优点。本研究通过对空肠弯曲菌及结肠弯曲菌16s rRNA基因相同保守序列的扩增, 来实现对样品中空肠弯曲菌及结肠弯曲菌的检测!试验结果表明!该方法具有较强的特异性及灵敏性能够直接从鸡肉样品中直接检测出这两种弯曲菌,具有较强的实际应用价值。
ATCC菌种：空肠弯曲菌
Campylobacter jejuni subsp. jejuni (Jones et al.) Veron and Chatelain拉丁名
(ATCC? 33291?)统一编号
Strain Designations 菌株别名 : AS-83-79 /
Type Strain 模式菌株 : no /
Biosafety Level 生物安全等级: 2
Deposited As Campylobacter fetus subsp. jejuni Smibert
Strain Designations 菌株别名 AS-83-79
Application 用途 Control strain Media testing
Enteric Research
Emerging infectious disease research
Isolation 分离基物 Human feces, Colorado
Type Strain 模式菌株no
Biosafety Level 生物安全等级 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 保藏方法
Frozen（冷冻物）: -80℃ or colder
Freeze-Dried（冻干物）: 2℃ to 8℃
Live Culture（活物）: See Propagation Section
Preceptrol? yes
Type Strain 模式菌株 no
Medium 培养基 ATCC? Medium 1115: Brucella albimi broth
ATCC? Medium 177: Fluid thioglycollate medium
ATCC? Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Growth Conditions 生长条件
Temperature 培养温度 : 37℃
Atmosphere 需氧情况 : Microaerophilic, 3-5% O2-10% CO2
Name of DepositoR 寄存人 PG Martin
Chain of Custody 来源国家 ATCC <-- PG Martin <-- WLL Wang
Isolation 分离基物 Human feces, Colorado
References 参考文献 Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3.
Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003.
Microbiology of food and animal feeding stuffs --- Horizontal method for detection and enumeration of Campylobacter spp. --- Part 1: Detection method. London, UK:British Standards Institution;British Standard BS EN ISO 10272-1:2006.
Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.
ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions)
Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM.
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