ATCC medium: 1849 Harpo's HTYEM (marine) medium
Trypticase Peptone (BD 211921)......5.0 g
Yeast extract.......................2.0 g
HEPES...............................4.0 g
Artificial Seawater (see below).....1.0 L
Adjust for final pH 7.5 with NaOH. Autoclave at 121C for 15 minutes. Artificial Seawater:
NaCl................................27.5 g
MgCl2 . 6H2O ........................5.38 g
MgSO4 . 7H2O ........................6.78 g
KCl.................................0.72 g
NaHCO3 ..............................0.2 g
CaCl2 . 2H2O ........................1.4 g
Distilled water.....................1.0 L