ATCC medium: 826 YGC Medium with glutamic acid
Yeast extract...............10.0 g
Glucose.....................20.0 g
CaCO3 .......................20.0 g
Glutamic acid................0.1 g
Agar........................20.0 g
Distilled water..............1.0 L
Autoclave at 10 lbs. for 30 minutes.
Place tubes in 48C water bath. Resuspend calcium carbonate by vibrating individual tubes and slant.