ATCC Medium: 416 Lactobacilli MRS Agar/Broth
Agar Medium
Lactobacilli MRS Agar (BD 288210)…………………..…..70 g
DI Water……………………...………………………………1000 ml
Boil to dissolve agar. Autoclave at 121?C.
Broth Medium
Lactobacilli MRS Broth (BD 288130)…………….55.0 g
DI Water……………………………..…………..….1000 ml
Autoclave at 121?C.
*This product is commercially available from BD
To make medium from scratch, follow formulation below
: Lactobacilli MRS Broth Composition
Proteose Peptone #3……………………………….10.0 g
Beef Extract………………………………………….10.0 g
Yeast Extract………………………………………...5.0 g
Dextrose……………………………………………..20.0 g
Sorbitan Monooleate……………………………….1.0 g
Ammonium Citrate………………………………….2.0 g
Sodium Acetate……………………………………..5.0 g
MnSO4 x H2O……………………………………….0.05 g
Na2HPO4……………………………………………2.0 g
DI Water……………………………………………..1000 m
l Final pH 6.5 +/- 0.2