ATCC medium: 793 Cytophaga agarase medium
KH2PO4 .....................................................1.0 g
NH4Cl ......................................................0.5 g
MgSO4 . 7H2O ...............................................0.5 g
CaCl2 . H2O ................................................0.02 g
Vishniac and Santer Trace Element Solution (see below).....0.2 ml
Deionized water............................................1.0 L
For broth add 1.0 g/L agar and for plates add 15.0 g/L agar. Adjust pH
to 7.2.
Autoclave at 121C for 15 minutes.
Vishniac and Santer Trace Element Solution:
EDTA..............................50.0 g
ZnSO4 . 7H2O ......................22.0 g
CaCl2 ..............................5.54 g
MnCl2 . 4H2O .......................5.06 g
FeSO4 . 7H2O .......................4.99 g
(NH4)6Mo7O24 . 4H2O..................1.10 g
CuSO4 . 5H2O .......................1.57 g
CoCl2 . 6H2O .......................1.61 g
Distilled water....................1.0 L
Adjust pH to 6.0 with KOH.