ATCC Medium: 2714 Middlebrook 7H9 Broth w/ ADC Enrichment
and 40mM Sodium Pyruvate
Middlebrook 7H9 Broth………………………………4.7 g
Glycerol………………………………………………..2.0 ml
Sodium pyruvate……………………………………..4.4 g
DI Water……………………………………………….900.0 ml
Add 4.7 grams of dehydrated Middlebrook 7H9 medium and 4.4 grams Sodium
pyruvate to 900 ml of water containing 2.0 ml of glycerol. Mix well and autoclave
at 121?C. Cool to 50-55?C.
Aseptically add 100 ml of the following filter sterilized ADC Enrichment:
ADC Enrichment Formulation
Bovine Serum Albumin, Frac V………………………5.0 g
Dextrose………………………………………………...2.0 g
Catalase(beef)………………………………………….3 mg
DI Water………………………………………………....100 ml