ATCC medium: 1717 Buffered S&H medium
Peptone............................5.0 g
Yeast extract......................5.0 g
Na2HPO4 ............................2.7 g
Citric acid . H2O ..................1.15 g
50% Glucose (filter-sterilized)...40.0 ml
Agar (if necessary)...............15.0 g
Distilled water..................960.0 ml
Adjust medium for final pH 5.0 with HCl. For solid medium, autoclave agar separately. Autoclave at 121C for 15 minutes. Aseptically add filter-sterilized glucose solution.