ATCC medium: 1957 Geobacter medium
NH4Cl ....................................1.5 g
NaH2PO4 ..................................0.6 g
KCl......................................0.1 g
NaHCO3 ...................................2.5 g
Sodium acetate...........................0.82 g
Sodium fumarate (filter-sterilized)......8.0 g
Wolfe's Vitamin Solution (see below)....10.0 ml
Modified Wolfe's Minerals (see below)...10.0 ml
Distilled water to.......................1.0 L
Prepare and dispense medium without fumarate anaerobically under an atmosphere of 80% N2, 20% CO2. Autoclave at 121C for 15 minutes. Add fumarate from a filter-sterilized, nitrogen-sparged stock solution prior to inoculation. Final pH of medium should be approximately 6.8. Wolfe's Vitamin Solution: Available from ATCC as a sterile ready-to-use liquid (Vitamin
Supplement, catalog no. MD-VS).
Biotin.......................2.0 mg
Folic acid...................2.0 mg
Pyridoxine hydrochloride....10.0 mg
Thiamine . HCl...............5.0 mg
Riboflavin...................5.0 mg
Nicotinic acid...............5.0 mg
Calcium D-(+)-pantothenate...5.0 mg
Vitamin B12..................0.1 mg
p-Aminobenzoic acid..........5.0 mg
Thioctic acid................5.0 mg
Distilled water..............1.0 L
Modified Wolfe's Minerals:
Na2SeO3 .................................10.0 mg
NiCl2 . 6H2O ............................10.0 mg
Na2WO4 . 2H2O............................10.0 mg
Wolfe's Mineral Solution (see below).....1.0 L