ATCC Medium: 2515 ? Strength Marine Medium
Marine Broth 2216 (BD 279110)………...…18.7 g
Agar (if needed)………………………...……15.0 g
DI Water…………………………….…….…...1000.0 ml
Autoclave at 121?C. Keep medium spinning while dispensing so that precipitate is evenly distributed throughout the medium.