ATCC medium: 2062 Desulfitobacterium frappieri medium
KH2PO4 .............................0.27 g
K2HPO4 .............................0.35 g
NH4Cl ..............................0.53 g
MgCl2 . 6H2O .......................0.10 g
FeCl2 . 4H2O .......................2.0 mg
CaCl2 . 2H2O ......................73.0 mg
SL-6 Trace Elements (see below)....1.0 ml
Resazurin..........................1.0 mg
Yeast extract......................1.0 g
Sodium sulfite.....................0.63 g
Sodium pyruvate....................6.05 g
Na2S . 9H2O ........................0.05 g
Distilled water....................1.0 L
Adjust medium, if necessary, for final pH 7.5. Prepare and dispense medium anaerobically under an atmosphere of 80% N2, 10% CO2, and 10% H2.
Autoclave at 121C for 15 minutes.
SL-6 Trace Elements:
ZnSO4 . 7H2O .................0.10 g
MnCl2 . 4H2O .................0.03 g
H3BO3 ........................0.3 g
CoCl2 . 6H2O .................0.2 g
CuCl2 . 2H2O .................0.01 g
NiCl2 . 6H2O .................0.02 g
Na2MoO4 . 2H2O................0.03 g
Distilled water..............1.0 L