ATCC medium: 1465 Basal thermophile medium
中国微生物菌种查询网 / 2017-01-06 16:01:30

ATCC medium: 1465 Basal thermophile medium

Solution 1:

NH4Cl ....................................0.9 g

MgCl2 . 6H2O .............................0.2 g

KH2PO4 ...................................0.75 g

K2HPO4 ...................................1.5 g

Trace Element Solution (see below).......9.0 ml

10% FeSO4 . 7H2O .........................0.03 ml

0.2% Resazurin...........................1.0 ml

Wolfe's Vitamin Solution (see below).....5.0 ml

Trypticase Peptone (BD 211921)..........10.0 g

Distilled water........................850.0 ml

Solution 2:

Yeast extract............................3.0 g

Distilled water........................100.0 ml

Solution 3:

Glucose..................................5.0 g

Distilled water.........................50.0 ml

Autoclave the above solutions separately at 121C for 45 minutes.

Combine solutions under 100% N2 and dispense aseptically using the same gas phase.

At time of inoculation add 10% sterile Na2S . 9H2O to a final concentration of 0.1%.

Trace Element Solution:

Nitrilotriacetic acid (Neutralize to pH 6.5 with KOH).....12.5 g

FeCl3 . 4H2O ...............................................0.2 g

MnCl2 . 4H2O ...............................................0.1 g

CoCl2 . 6H2O ...............................................0.017 g

CaCl2 . 2H2O ...............................................0.1 g

ZnCl2 ......................................................0.1 g

CuCl2 ......................................................0.02 g

H3BO3 ......................................................0.01 g

Na2MoO4 . 2H2O..............................................0.01 g

NaCl.......................................................1.0 g

Na2SeO3 ....................................................0.02 g

Distilled water............................................1.0 L

2 Wolfe's Vitamin Solution:

Available from ATCC as a sterile ready-to-use liquid (Vitamin Supplement, catalog no. MD-VS).

Biotin.......................2.0 mg

Folic acid...................2.0 mg

Pyridoxine hydrochloride....10.0 mg

Thiamine . HCl...............5.0 mg

Riboflavin...................5.0 mg

Nicotinic acid...............5.0 mg

Calcium D-(+)-pantothenate...5.0 mg

Vitamin B12..................0.1 mg

p-Aminobenzoic acid..........5.0 mg

Thioctic acid................5.0 mg

Distilled water..............1.0 L

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