ATCC Medium: 2495 10mM Phosphate Buffer
Sodium Phosphate Monobasic Monohydrate…………………….8.36g
Sodium Phosphate Dibasic Anhydrous…………………………...19.6g
DI Water……………………………………………………………….10L
Combine and check pH which should be 7.2 +/- 0.2. If the pH is not within this range the material must be discarded and remade (the acid and base used to pH will affect the dye used by the RBD 3000 making the testing invalid).
Filter Sterilize.