ATCC Medium: 2813 DNB Medium
Nutrient Broth……………………0.8 g
100 mM CaCl2 . 2H2O.…………..10 ml
10 mM MgCl2 . 6H2O….…………10 ml
DI Water…………………………980 ml
Adjust pH to 7.2 with 1 N NaOH. Autoclave at 121C.
For bottom layer add 1.5 % agar (this layer will be dispensed into a plate).
For top layer add 0.8% agar (this layer will be dispensed into screw cap tubes).