ATCC medium: 1541 Modified Singh's medium
NaH2PO4 . H2O.............................0.250 g
MgCl2 . 6H2O .............................0.125 g
KCl......................................0.250 g
CaCl2 . 2H2O .............................0.250 g
NaCl.....................................8.750 g
D-Glucose................................5.000 g
Lactalbumin hydrolysate..................8.125 g
TC Yeastolate (BD 255772)................6.250 g
NaHCO3 ...................................0.150 g
Fetal bovine serum (heat-inactivated)..200.0 ml
Phenol red..............................10.00 mg
Distilled water........................800.0 ml
Adjust medium, if necessary, to pH 7.0 with NaOH. Filter-sterilize.