ATCC Medium: 1705 Brucella Agar/Broth with 5% Defibrinated Sheep Blood
Agar Medium
Brucella Agar (BD 211086)…………………..……...43.0 g
Sheep Blood (defibrinated; aseptically added)…....50 ml
DI Water…………………………………………..…...950 ml
Autoclave at 121?C. Cool sterilized medium to ~47?C. Aseptically add 50 ml of room temperature defibrinated sheep blood. Gently mix and dispense as required.
Broth Medium
Brucella Broth (BD 211088)……………………….….28.0 g
Sheep Blood (defibrinated; aseptically added)…......50 ml
DI Water………………………………………..…...…..950 ml
Autoclave at 121?C. Cool sterilized medium to ~47?C. Aseptically add 50 ml of room temperature defibrinated sheep blood. Gently mix and dispense as required.