ATCC Medium: 111 Rhizobium X Medium
Yeast Extract..................................................................1.0 g
Mannitol..........................................................................10.0 g
Agar................................................................................15.0 g
Soil Extract (see below)…………………………………..200.0 ml
Distilled water.................................................................800.0 ml
Adjust pH to 7.2. Autoclave at 121?C. Soil Extract
African violet soil.............................................................77.0 g
Na2CO3…………………………………….......................0.2 g
DI Water……………………………………………............200.0 ml
Autoclave for one hour. Filter through paper to remove soil before using in media.