Agar..........................................15.0 g
Tryptone......................................20.0 g
Tryptose.......................................5.0 g
Yeast extract..................................5.0 g
Tomato juice (filtered, pH 7.0)..............200.0 ml
Liver Extract Concentrate (ICN 100377).........1.0 g
Tween 80.......................................0.05 g
Glucose........................................3.0 g
Lactose........................................2.0 g
Distilled water to.............................1.0 L
Adjust pH to 6.5. Autoclave at 121C for 15 minutes.