ATCC Medium: 2174 Brain Heart Infusion Medium w/ 4mcg/ml Vancomycin
Base Medium
BHI Broth (BD 237500)…………..…………37.0 g
DI Water…………………………………...….950 ml
Autoclave at 121?C. Once base has cooled to 50?C, ascetically add the following: Vancomycin Solution
Vancomycin…………………………………..0.004 g
DI Water……………………………………….50 ml
*If the reagent antibiotic powder indicates an assay value of potency, use that value to correctly calculate the amount of powder needed. Filter sterilize.
*To make agar medium replace BHI Broth with 52 grams of BHI Agar (BD 211065)