Agar Medium
Heart Infusion Agar (BD 244400)….............40 g
DI Water……………………………………1000 ml
Autoclave at 121? C.
*This product is commercially available from BD.
To make medium from scratch, follow formulation below:
Heart Infusion Agar Composition
Beef Heart, Infusion from 500g……………...10.0g
Tryptose………………………………………10.0 g
Sodium Chloride……………………………...5.0 g
Agar…………………………………………...15 g
Adjust pH to 7.4 +/- 0.2
Broth Medium
Heart Infusion Broth (BD cat 238400)…………….25.0 g
DI Water………………………………………..….1000 ml
Autoclave at 121? C.
*This product is commercially available from BD.
To make medium from scratch, follow same formulation as above but omit agar