Complete Medium
*YPD Broth (BD 242820)……………………….………..50 g
Geneticin Solution (add aseptically, see below)…..…..50 ml
DI Water………………………………………………..….950 ml
*This product is commercially available from BD.
To make agar product, use 65 g of YPD Agar (BD 242720) in place of YPD Broth.
Geneticin Solution
Geneticin…………………………………………………0.2 g
DI Water……………………………………………….….50 ml
Add to base medium aseptically.
Note: If commercial product is unavailable, make YPD from scratch, following the formulation below:
Yeast Extract........................................................10.0 g
Peptone................................................................20.0 g
Dextrose...............................................................20.0 g
Agar.....................................................................15.0 g
DI Water…………………………………………….1000 ml
Combine ingredients, heat to dissolve agar if necessary, and autoclave at 121?C.