ATCC medium: 731 Luminous medium
NaCl..............................30.0 g
K2HPO4 .............................3.9 g
KH2PO4 .............................2.1 g
NH4Cl ..............................5.0 g
Yeast extract......................5.0 g
Tryptone...........................5.0 g
1 M Tris buffer, pH 7.5...........50.0 ml
Glycerol...........................3.0 ml
MgSO4 . 7H2O .......................1.0 g
KCl................................0.75 g
CaCO3 ..............................1.0 g
Agar (if needed)..................20.0 g
Distilled water to.................1.0 L
Adjust pH to 7.2.
Autoclave 15 minutes at 121C.