ATCC medium: 1659 YA halophile medium
Na2HPO4 ......................3.8 g
KH2PO4 .......................1.3 g
(NH4)2SO4.....................1.0 g
NaCl.......................100.0 g
Sodium acetate . 3H2O .......10.0 g
Yeast extract................1.0 g
Mg(NO3)2 . 6H2O*..............1.0 g
Agar........................15.0 g
Distilled water to...........1.0 L
Adjust medium for final pH 7.2 with KOH.
Autoclave at 121C for 15 minutes. *Add aseptically after sterilization of medium.