ATCC medium: 1342 Halobacteroides medium
NaCl..............................88.0 g
MgCl2 . 6H2O ......................20.0 g
CaCl2 . 2H2O .......................7.4 g
KCl................................3.7 g
Yeast extract......................5.0 g
Resazurin..........................1.0 mg
Distilled water to...............900.0 ml
Boil under nitrogen, add 0.5 g L-cysteine . HCl, and autoclave anaerobically at 121C, 15 minutes. Cool medium under nitrogen and add aseptically the following anaerobically prepared sterile solutions:
10% Glucose.......................50.0 ml
1 M PIPES Na2 salt, pH 6.8-7.0 ....40.0 ml
Dispense medium anaerobically.